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Regulation of collagen production in freshly isolated cell populations from normal and cirrhotic rat liver: Effect of lactate
Author(s) -
CerbónAmbriz Jorge,
CerbónSolórzano Jorge,
Rojkind Marcos
Publication year - 1991
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840130324
Subject(s) - lactic acid , hydroxyproline , ccl4 , incubation , cirrhosis , medicine , endocrinology , hepatic stellate cell , proline , chemistry , hepatocyte , biochemistry , biology , in vitro , carbon tetrachloride , amino acid , bacteria , genetics , organic chemistry
Previous work has shown that lactic acid, and to a lesser extent pyruvic acid, is able to increase collagen synthesis significantly in liver slices of CCl 4 ‐treated rats but not normal rats. The purpose of this report is to document which cells in the cirrhotic liver are responsible for the lactate‐stimulated increase in collagen synthesis. It was found that (a) incorporation of 3 H‐proline into protein‐bound 3 H‐hydroxyproline is increased threefold to fourfold in hepatocytes from CCl 4 ‐treated rats as compared with normal rat hepatocytes; (b) neither the hepatocytes from normal nor those from CCl 4 ‐treated rats modify their collagen synthesizing capacity when 30 mmol/L lactic acid was added to the incubation medium; (c) nonparenchymal cells obtained from livers of CCl 4 ‐treated rats synthesize much less collagen than hepatocytes, but their synthesis is stimulated twofold by lactic acid; (d) from the different nonparenchymal cells, only fat‐storing (Ito) cells increase collagen synthesis when lactic acid is present in the incubation medium. These results suggest that the increased lactic acid levels observed in patients with alcoholic hepatic cirrhosis may play an important role in the development of fibrosis by stimulating collagen production by fat‐storing (Ito) cells. (H EPATOLOGY 1991;13:551–556.)