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Localization of epidermal growth factor receptor in hepatocyte nuclei
Author(s) -
Marti Ulrich,
Burwen Susan Jo,
Wells Alan,
Barker Mary E.,
Huling Sandra,
Feren Anna M.,
Jones Albert L.
Publication year - 1991
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840130104
Subject(s) - epidermal growth factor , epidermal growth factor receptor , tgf alpha , growth factor receptor inhibitor , biology , hepatocyte growth factor receptor , growth factor receptor , receptor , microbiology and biotechnology , hepatocyte , liver receptor homolog 1 , endocrinology , medicine , chemistry , hepatocyte growth factor , biochemistry , nuclear receptor , in vitro , c met , transcription factor , gene
Experiments undertaken to investigate the binding of epidermal growth factor by hepatocyte nuclei showed that: (a) isolated nuclei from both normal and regenerating rat liver are capable of binding 125 I‐epidermal growth factor, (b) the nuclear epidermal growth factor‐binding protein is similar in molecular weight to the plasma membrane epidermal growth factor receptor, (c) monoclonal antibodies produced against the plasma membrane epidermal growth factor receptor recognize the nuclear epidermal growth factor receptor and (d) the nuclear receptor has an affinity for epidermal growth factor comparable to that of the plasma membrane receptor, but fewer (∼ 10%) nuclear receptors are available per protein unit compared with the plasma membrane. (HEPATOLOGY 1991;13:15–20).