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Antibodies to nuclear lamin C in chronic hepatitis delta virus infection
Author(s) -
WȩsierskaGa̧dek Józefa,
Penner Edward,
Hitchman Eva,
Sauermann Georg
Publication year - 1990
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840120509
Subject(s) - lamin , nuclear lamina , biology , immunofluorescence , cell nucleus , antibody , epitope , virology , autoantibody , virus , antigen , nuclear protein , microbiology and biotechnology , immunology , nucleus , genetics , gene , transcription factor
Sera of patients with chronic hepatitis delta virus infection stained the nuclear periphery in indirect immunofluorescence. Using proteins of isolated nuclei, isolated nuclear matrices, the nuclear pore complexlamina fraction and purified lamins A and C as antigen source in immunoblotting experiments, nuclear lamin C was identified as the reactive antigen. Most sera tested (8 of 10) recognized nuclear lamin C exclusively, but not the nuclear lamins A and B. Antibodies reacting with both nuclear lamins A and C, which share extensive sequence homologies, have been reported to occur in autoimmune hepatitis and primary biliary cirrhosis. The present findings suggest that the novel autoantibody associated with chronic hepatitis delta virus infection recognizes an epitope localized in the short carboxyterminal region of nuclear lamin C. (HEPATOLOGY 1990;12:1129–1133).