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Uptake of thymidine labeled on carbon 2: A potential index of liver regeneration by positron emission tomography
Author(s) -
Borght Thierry M. Vander,
Lambotte Luc E.,
Pauwels Stanislas A.,
Dive Charles C.
Publication year - 1990
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840120118
Subject(s) - thymidine , positron emission tomography , chemistry , liver regeneration , fractionation , dna , radiochemistry , tracer , regeneration (biology) , biochemistry , chromatography , nuclear medicine , biology , medicine , microbiology and biotechnology , physics , nuclear physics
Noninvasive measurement of liver regeneration with positron emission tomography has been attempted with 11 C‐thymidine; however, results were unsatisfactory using thymidine labeled on its methyl group. To evaluate whether the specificity of the method could be improved by modifying the labeling position of the tracer, thymidine labeled on its methyl group with 3 H and thymidine labeled on its carbon 2 with 14 C were injected in 22 hepatectomized rats either 1 hr (when DNA synthesis is not increased) or 24 hr after the surgical procedure (when the rate of DNA synthesis is maximal). Liver samples taken 10, 30 and 120 min after injection showed that, in contrast to 3 H‐radioactivity, 14 C‐radioactivity measured in whole tissue allowed a clear discrimination between regenerating and nonregenerating livers. In addition, 14 Cradioactivity measured in whole tissue of regenerating livers correlated with the DNA radioactivity 10, 30 and 120 min after injection of the tracer. In contrast, no such correlation was found with the methyl‐labeled thymidine. Analysis of the radioactive material present in the nonDNA fraction using ion exchange disks and high‐performance liquid chromatography showed that 2‐C–labeled thymidine was incorporated into DNA without accumulation of labeled metabolites whereas, for the methyl‐labeled thymidine, almost all radioactivity was related to degradative products. Therefore the evaluation of the liver regeneration with the 2‐C–labeled thymidine, which does not require cellular fractionation, should be suited for noninvasive measurement with positron emission tomography. (H EPATOLOGY 1990;12:113–118).