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Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats
Author(s) -
Kato Shinzo,
Ishii Hiromasa,
Aiso Sadakazu,
Yamashita Shuji,
Ito Daisuke,
Tsuchiya Masaharu
Publication year - 1990
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840120111
Subject(s) - immunohistochemistry , alcohol dehydrogenase , alcohol , acinus , staining , biology , lobules of liver , alcohol oxidoreductase , lactate dehydrogenase , distribution (mathematics) , biochemistry , enzyme , medicine , chemistry , pathology , endocrinology , nad+ kinase , pancreas , mathematical analysis , mathematics
The distribution of alcohol dehydrogenase in the hepatic acinus was examined by both histochemical and immunohistochemical approaches. The immunohistochemical method using anti–alcohol dehydrogenase antibody indicated zone 3 predominance of this enzyme in the hepatic acinus, whereas a conventional histochemical method showed slight zone 1 predominance. However, when the histochemical technique was improved by using 2% glutaraldehyde instead of formalin for fixation and by adding phenazine methosulfate (0.33 mmol/L) to the staining incubation mixture, this method also supported zone 3 predominance of alcohol dehydrogenase. Evidence for zone 3 distribution of alcohol dehydrogenase may be of value in elucidating the mechanism of zone 3 liver damage by alcohol. (H EPATOLOGY 1990;12:66–69).