Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats | Zendy

Kato Shinzo | Zendy; Ishii Hiromasa | Zendy; Aiso Sadakazu | Zendy; Yamashita Shuji | Zendy; Ito Daisuke | Zendy; Tsuchiya Masaharu | Zendy

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Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats

Author(s) -

Kato Shinzo,

Ishii Hiromasa,

Aiso Sadakazu,

Yamashita Shuji,

Ito Daisuke,

Tsuchiya Masaharu

Publication year - 1990

Publication title -

hepatology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.488

H-Index - 361

eISSN - 1527-3350

pISSN - 0270-9139

DOI - 10.1002/hep.1840120111

Subject(s) - immunohistochemistry , alcohol dehydrogenase , alcohol , acinus , staining , biology , lobules of liver , alcohol oxidoreductase , lactate dehydrogenase , distribution (mathematics) , biochemistry , enzyme , medicine , chemistry , pathology , endocrinology , nad+ kinase , pancreas , mathematical analysis , mathematics

The distribution of alcohol dehydrogenase in the hepatic acinus was examined by both histochemical and immunohistochemical approaches. The immunohistochemical method using anti–alcohol dehydrogenase antibody indicated zone 3 predominance of this enzyme in the hepatic acinus, whereas a conventional histochemical method showed slight zone 1 predominance. However, when the histochemical technique was improved by using 2% glutaraldehyde instead of formalin for fixation and by adding phenazine methosulfate (0.33 mmol/L) to the staining incubation mixture, this method also supported zone 3 predominance of alcohol dehydrogenase. Evidence for zone 3 distribution of alcohol dehydrogenase may be of value in elucidating the mechanism of zone 3 liver damage by alcohol. (H EPATOLOGY 1990;12:66–69).

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