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Extrahepatic replication of duck hepatitis b virus: More than expected
Author(s) -
Hosoda Kazuhiko,
Omata Masao,
Uchiumi Katsuo,
Imazeki Fumio,
Yokosuka Osamu,
Ito Yoshimi,
Okuda Kunio,
Ohto Masao
Publication year - 1990
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840110109
Subject(s) - virology , replication (statistics) , duck hepatitis b virus , hepatitis b virus , virus , medicine , biology , hepadnaviridae
Replication of duck hepatitis B virus in extrahepatic tissue such as pancreas, kidney and spleen has been well documented. To assess whether there is more widespread extraheptic virus replication, we assayed brain, heart, lung, thymus, pancreas, kidney, spleen and intestine of 1‐ to‐wk‐old ducklings for the presence of duck hepatitis B virus DNA and mRNA by blotting in in situ methods. Replicative intermediates and single‐stranded duck hepatitis B virus DNA and RNA transcripts were detected in the brain, lung, heart, intestine, kidney, pancreas and spleen. In situ hybridization showed evidence of viral replication in the lung epithelium, germinal center of spleen, acinar cell of pancreas and tubular epithelium of kidney. These data suggest that extrahepatic duck hepatitis B virus replication is more widespread than previously thought. It is yet to be determined whether widespread extrahepatic replication is unique to duck hepatitis B virus infection or is a common feature of other mammalian hepatitis B‐like viruses. (HEPATOLOGY 1990; 11: 44–48.)