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The integrated value of serum procollagen III peptide over time predicts hepatic hydroxyproline content and stainable collagen in a model of dietary cirrhosis in the rat
Author(s) -
Ruwart Mary J.,
Wilkinson Karen F.,
Rush Bob D.,
Vidmar Thomas J.,
Peters Kenneth M.,
Henley Keith S.,
Appelman Henry D.,
Kim Kiyoung Y.,
Schuppan Detlef,
Hahn Eckert G.
Publication year - 1989
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840100509
Subject(s) - hydroxyproline , procollagen peptidase , medicine , endocrinology , cirrhosis , hepatic fibrosis , chemistry , prostaglandin e2 , fibrosis , peptide , histopathology , prostaglandin e , type i collagen , pathology , biochemistry
To determine whether a serum parameter of collagen metabolism, serum procollagen type III peptide, correlated with hepatic collagen in a model of diet‐induced fibrosis, rats were fed a control or cirrhogenic diet for 6 months and treated with either subcutaneous vehicle or the hepatoprotective prostaglandin 16,16‐dimethyl prostaglandin E2 (100 μg per kg) twice daily. Pair‐fed rats from each group were killed after 2, 4 or 6 months. The value of serum procollagen type III peptide to body weight integrated over time (K t ) correlated linearly with hepatic hydroxyproline content (r = 0.97) at killing time t. Good correlations were also seen between K t and histopathological assessment of aniline blue‐stainable collagen (r = 0.93) and between the histopathology and hydroxyproline content (r = 0.97). Rats receiving 16,16‐dimethyl prostaglandin E 2 had lower values of all three parameters compared to rats receiving vehicle, confirming the previously demonstrated hepatoprotective effect of 16,16‐dimethyl prostaglandin E 2 . The excellent correlation between K t and the two other traditional parameters of hepatic collagen suggest that sequential measurements of serum procollagen type III peptide can be used to predict alterations in liver collagen deposition in rats.