Premium
Ubiquitin—a common denominator in intermediate filament pathology of brain and liver?
Author(s) -
Denk Helmut,
Zatloukal Kurt,
Preisegger KarlHeinz
Publication year - 1989
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840100419
Subject(s) - ubiquitin , inclusion bodies , pick's disease , pathology , intermediate filament , pathogenesis , cytoplasmic inclusion , cytoplasm , biology , polyclonal antibodies , cytochemistry , disease , antibody , microbiology and biotechnology , medicine , ultrastructure , immunology , biochemistry , dementia , cytoskeleton , escherichia coli , gene , cell
Polyclonal antibodies were raised which have a high affinity for conjugated ubiquitin. Immuno‐cytochemistry was performed on paraffin sections of tissues showing well‐characterized inclusion bodies. Ubiquitin was found as a component of the intermediate filament inclusion bodies characteristic of several major diseases including Lewy bodies of Parkinson's disease, Pick bodies of Pick's disease, Mallory bodies of alcoholic liver disease, cytoplasmic bodies of a specific myopathy, and Rosenthal fibres within astrocytes. Ubiquitin was also present in the three histological lesions characteristic of Alzheimer's disease. These observations suggest a fundamental role for ubiquitin in the formation of intermediate filament inclusion bodies in man, and have implications regarding the pathogenesis of these important diseases.