Premium
Binding and uptake of native and modified low‐density lipoproteins by human hepatocytes in primary culture
Author(s) -
Babaev Vladimir R.,
Kosykh Vladimir A.,
Tsibulsky Vladimir P.,
Ivanov Vadim O.,
Repin Vadim S.,
Smirnov Vladimir N.
Publication year - 1989
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840100112
Subject(s) - malondialdehyde , hepatocyte , low density lipoprotein , chemistry , lipoprotein , biochemistry , microbiology and biotechnology , biology , cholesterol , oxidative stress , in vitro
The binding and uptake of native low‐density lipoproteins and malondialdehyde‐treated low density lipoproteins by human hepatocytes in primary culture has been analyzed. Experiments with 125 I‐labeled malondialdehyde‐treated low‐density lipoproteins showed that cultured liver cells took up and degraded malondialdehyde‐treated low‐density lipoproteins, but the cell type(s) responsible for this action remain unclear. Immunofluorescent visualization of receptor‐bound low‐density lipoproteins revealed that low‐density lipoprotein binding sites were distributed on the surface of nearly all cells of the culture. Binding sites for malondialdehyde‐treated low‐density lipoproteins were found in only 5% of the cultured cells, and these cells differed from hepatocytes in shape and size. Cultured hepatocytes internalized and native low‐density lipoproteins, but not malondialdehyde‐treated low‐density lipoproteins, labeled with the fluorescent dye 3′,3′‐dioctadecylindocarbocyanine. About 15% of the cells that take up 3′,3′‐dioctadecylindocarbocyanine‐labeled malondialdehyde‐treated low‐density lipoproteins could be identified as liver endothelial cells and macrophages, since they internalized formaldehyde‐treated human albumin and fluorescent carboxylated microspheres. Our results indicate that human hepatocytes in primary culture express surface receptors for native low‐density lipoproteins but not for modified low‐density lipoproteins.