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Studies on the regulation of cholesterol metabolism by low‐and high‐density lipoproteins in HepG2 cellsx
Author(s) -
Ranganathan Subramanian,
Kottke Bruce A.
Publication year - 1989
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840090407
Subject(s) - cholesterol , reverse cholesterol transport , intermediate density lipoprotein , metabolism , chemistry , high density lipoprotein , biochemistry , sterol o acyltransferase , efflux , very low density lipoprotein , lipoprotein , medicine , endocrinology , biology
The uptake and degradation of low‐density lipoproteins and the esterification and synthesis of cholesterol were poorly down‐regulated by low‐density lipoproteins in HepG2 cells. Addition of low‐density lipoproteins to the cells increased the free and esterified cholesterol in the cells. The heavier fraction of high‐density lipoproteins enhanced the degradation of low‐density lipoproteins and cholesterol synthesis and decreased acyl CoA:cholesterol acyltransferase activity. Addition of the heavier fraction of high‐density lipoproteins also caused a net efflux of cholesterol from HepG2 cells. The lighter fraction did not have any significant effect on cholesterol metabolism or cellular cholesterol level. Neither the lighter nor the heavier fractions of high‐density lipoproteins were found to have any specific binding properties to HepG2 cells.