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Differential regulation of glutathione S‐transferases in cultured hepatocytes
Author(s) -
Abramovitz Mark,
Ishigaki Seishi,
Listowsky Irving
Publication year - 1989
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840090212
Subject(s) - hepatocyte , glutathione , messenger rna , cell culture , biology , microbiology and biotechnology , gene expression , transcription (linguistics) , isozyme , complementary dna , gene , enzyme , biochemistry , in vitro , genetics , linguistics , philosophy
Specific cDNA probes were used to determine steady‐state mRNA levels for the multiple glutathione S‐trans‐ferases in primary hepatocyte cultures. In the first 24 hr of culture, gene transcripts for the Y a family decreased sharply, Y b3 disappeared completely, but changes in levels of mRNA for Y b1 and Y b2 were smaller. These results suggest that the isoenzymes are regulated independently. Y p mRNA, which is present at greatly elevated levels in hyperplastic nodules and hepatocel‐lular carcinomas but not in normal adult livers, was hardly detectable in freshly isolated hepatocytes, but Y p transcripts rapidly accumulated in the first 24 hr in culture and continued to increase for 72 hr. Decreased levels in Y a and Y c and increases in Y p were detected by immunoblotting methods, indicating that translation products changed together with mRNA levels in the cultured cells. The appearance of Y p transcripts in hepatocytes was effectively blocked by addition of dexa‐methasone to the culture medium. Elevations of Y p levels are characteristic of the cell culture system and factors regulating Y p transcription in nodules and carcinomas may also be operative in cultured hepatocytes.