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Antibody to liver cytosol (anti‐LC1) in patients with autoimmune chronic active hepatitis type 2
Author(s) -
Martini Eric,
Abuaf Nisen,
Cavalli Florence,
Durand VÉRonique,
Johanet Catherine,
Homberg JeanClaude
Publication year - 1988
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840080632
Subject(s) - hepatitis , autoantibody , antibody , primary biliary cirrhosis , autoimmune hepatitis , antigen , immunofluorescence , immunology , medicine , pathology
A new autoantibody was detected by immunoprecipitation in the serum of 21 patients with chronic active hepatitis. The antibody reacted against a soluble cytosolic antigen in liver. The antibody was organ specific but not species specific and was therefore called antiliver cytosol antibody Type 1 (anti‐LC1). In seven of 21 cases, no other autoantibody was found; the remaining 14 cases had anti‐liver/kidney microsome antibody Type 1 (anti‐LKM1). With indirect immunofluorescence, a distinctive staining pattern was observed with the seven sera with anti‐LC1 and without anti‐LKM1. The antibody stained the cytoplasm of hepatocytes from four different animal species and spared the cellular layer around the central veins of mouse and rat liver that we have called juxtavenous hepatocytes. The immunofluorescence pattern disappeared after absorption of sera by a liver cytosol fraction. The 14 sera with both antibodies displayed anti‐LC1 immunofluorescent pattern after absorption of anti‐LKM1 by the liver micro‐somal fraction. The anti‐LC1 was found in the serum only in patients with chronic active hepatitis of unknown cause. Anti‐LC1 antibody was not found in sera from 100 patients with chronic active hepatitis associated with anti‐actin antibody classic chronic active hepatitis Type 1, 100 patients with primary biliary cirrhosis, 157 patients with drug‐induced hepatitis and a large number of patients with liver and nonliver diseases. This new antibody was considered a second marker of chronic active hepatitis associated with anti‐LKM1 (anti‐LKM1 chronic active hepatitis) or autoimmune chronic active hepatitis Type 2.