Premium
Serum 2′,5′‐oligoadenylate synthetase activity during interferon treatment of chronic hepatitis B
Author(s) -
Shindo Michiko,
Okuno Tadao,
Matsumoto Masayuki,
Takeda Makoto,
Takino Tatsuro,
Sokawa Junko,
Iwata Akira,
Sokawa Yoshihiro
Publication year - 1988
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840080229
Subject(s) - peripheral blood mononuclear cell , interferon , medicine , discontinuation , immunology , hepatitis c virus , endocrinology , biology , virus , in vitro , biochemistry
We measured 2′,5′‐oligoadenylate synthetase activities in serum and peripheral blood mononuclear cells from 10 patients with chronic hepatitis B who were being treated with interferon so as to determine whether 2′,5′‐oligoadenylate synthetase activity in serum reflected 2′,5′‐oligoadenylate synthetase activity in peripheral blood mononuclear cells, and whether it could be used to monitor interferon treatment. Pretreatment values of 2′,5′‐oligoadenylate synthetase activity in patients' serum and peripheral blood mononuclear cells were not statistically different from values from control subjects. When interferon was administered, serum levels of 2′,5′‐oligoadenylate synthetase began to rise within 3 hr, reached peak values at 12 hr and then declined. The levels of 2′,5′‐oligoadenylate synthetase activity both in serum and peripheral blood mononuclear cells increased substantially during interferon treatment, ranging 2‐ to 50‐fold greater than initial levels. The levels of 2′,5′‐oligoadenylate synthetase in serum correlated closely with levels in peripheral blood mononuclear cells. In addition, when the levels of 2′,5′‐oligoadenylate synthetase rose during interferon administration, serum hepatitis B virus DNA polymerase values fell, and, in some cases, DNA polymerase rose again when 2′,5′‐oligoadenylate synthetase fell after discontinuation of interferon. These findings' suggest that 2′,5′‐oligoadenylate synthetase activity in serum accurately reflects the antiviral effect of interferon and could be used to monitor interferon treatment.