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16,16‐dimethyl prostaglandin E 2 delays collagen formation in nutritional injury in rat liver
Author(s) -
Ruwart Mary J.,
Rush Bob D.,
Snyder Karen F.,
Peters Ken M.,
Appelman Henry D.,
Henley Keith S.
Publication year - 1988
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840080112
Subject(s) - hydroxyproline , prostaglandin e2 , prostaglandin e , prostaglandin , medicine , endocrinology , chemistry , subcutaneous injection
Chronic nutritional injury was induced in rats by a high‐fat, lipotrope‐deficient diet. The hepatoprotective effect of 16,16‐dimethyl prostaglandin E 2 on the deposition of collagen and fat was assessed by histological evaluation and measurement of hydroxyproline. Dose‐response studies established that optimal protection was achieved by the twice daily administration of 16,16‐dimethyl prostaglandin E 2 at 100 μg per kg (subcutaneous) or 250 μg per kg (oral). 16,16‐Dimethyl prostaglandin E 2 and a crystalline analog [( p ‐acetami‐dobenzamido)phenyl ester of 16,16‐dimethyl prostaglandin E 2 significantly delayed both the deposition of collagen and the increase in hepatic hydroxyproline content. There was an excellent correlation between the histological assessment of collagen and the biochemical measurement of hydroxyproline. These data provide a rationale for the evaluation of prostaglandins in the treatment of human liver disease.