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The in vitro production of Dane‐like particles: Is it live or memorex?
Author(s) -
Farraye Francis A.,
Zeldis Jerome B.
Publication year - 1987
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840070336
Subject(s) - in vitro , production (economics) , chemistry , business , economics , biochemistry , macroeconomics
Closed‐circular HBV DNA was introduced into cells of the established human hepatoma culture HepG2. The culture medium of one of 40 single‐cell clones contained HBV surface antigen (HBsAg), core‐related antigens (HBc/eAg), and HBV DNA sequences. HBV DNA and DNA polymerase activity were detected in particles resembling both nucleocapsids and complete virions (Dane particles). Intracellular integrated and extrachromosomal HBV DNA sequences were detected. Relaxed‐circular and single‐stranded forms of viral DNA were identified as likely replicative intermediates of the HBV genome. In conclusion, in vitro production of Dane‐like particles by transformed human hepatocytes has been achieved. This model should be valuable as a cell culture system for studying virus replication and virus‐host cells interactions.