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Decreased uptake of taurocholate and ouabain by hepatocytes isolated from cirrhotic rat liver
Author(s) -
Reichen Jürg,
Hoilien Catherine,
Le Mysan,
Jones Richard H.
Publication year - 1987
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840070115
Subject(s) - ouabain , cirrhosis , hepatocyte , medicine , carbon tetrachloride , endocrinology , trypan blue , chemistry , lactate dehydrogenase , cell , biology , biochemistry , enzyme , in vitro , organic chemistry , sodium
To differentiate between the “intact” and “sick” cell hypothesis explaining decreased clearance of endo‐ and xenobiotics, we measured uptake of taurocholate and ouabain in hepatocytes isolated from cirrhotic rat liver. Cirrhosis was induced by chronic exposure of male Sprague‐Dawley rats to phenobarbital and carbon tetrachloride. Uptake of [ 14 C]taurocholate and [ 3 H]ouabain was measured by a rapid filtration technique. Hepatocytes from cirrhotic liver were as viable as control hepatocytes—as judged by trypan blue exclusion and lactate dehydrogenase release—but consumed 28% less oxygen. V max of both taurocholate (3.16 ± 0.95 vs. 0.40 ± 0.35 nmoles·min −1 ·10 6 cells −1 ; p < 0.001) and ouabain (2.16 ± 0.78 vs. 0.83 ± 0.26 nmoles·min −1 ·10 6 cells −1 ; p < 0.005) was significantly reduced. These results are compatible with the “sick” cell hypothesis.