Premium
Effect of experimental liver disease on the utilization for protein synthesis of orally administered α‐ketoisocaproate
Author(s) -
Muñoz Santiago,
Walser Mackenzie
Publication year - 1986
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840060325
Subject(s) - medicine , leucine , endocrinology , carbon tetrachloride , cirrhosis , protein biosynthesis , biology , chemistry , biochemistry , amino acid , organic chemistry
The incorporation of orally administered 1‐ 14 C‐α‐ketoisocaproate into the leucine of proteins in rats was compared with the incorporation of [ 3 H]leucine itself administered simultaneously and expressed as a ratio, R. This ratio in whole body protein has been shown to be approximately equal to the nutritional efficiency of α‐ketoisocaproate as a dietary substitute for leucine. In normal rats on a 14% protein diet, R in whole body protein (0.30 ± 0.01) and in the protein of various organs was the same whether the isotopes were given by single injection or 6‐hr constant infusion. Thus, both techniques yield the same time‐independent parameter, R, which measures the relative efficiency of α‐ketoisocaproate as a substitute for leucine. R varied between organs as follows: liver (0.22 ± 0.01)