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Establishment of a cell line from a woodchuck hepatocellular carcinoma
Author(s) -
Unoura Masashi,
Kobayashi Kenichi,
Fukuoka Kenichi,
Matsushita Fumiaki,
Morimoto Hideo,
Oshima Tohru,
Kaneko Shuichi,
Hattori Nobu,
Murakami Seishi,
Yoshikawa Hiroshi
Publication year - 1985
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840050608
Subject(s) - woodchuck hepatitis virus , hepatocellular carcinoma , biology , cell culture , microbiology and biotechnology , virology , hepatitis b virus , hepadnaviridae , virus , cancer research , genetics
Abstract A new cell line derived from a woodchuck hepatocellular carcinoma serially transplanted in athymic nude mice has been established and named WH257GE10. The original tumor in the nude mouse system produces woodchuck hepatitis surface antigen and albumin. In addition, woodchuck hepatitis virus DNA is integrated into cellular DNA. Adaptation of the cells to the in vitro culture condition was completed after 15 months with the doubling time of 40 hr. The morphologic features of the cell by light microscopy are of an epithelial type. The modal chromosome number is 36 and the karyotype is mainly metacentric, similar to that observed in normal woodchuck liver cells. Ornithine and tyrosine aminotransferase activities were detected. Production of albumin was demonstrated in the cytoplasm by indirect immunofluorescence. Integration of woodchuck hepatitis virus DNA was shown by Southern blot analysis, although the secretion of woodchuck hepatitis surface antigen was not detected. This cell line provides an excellent in vitro model to study human hepatocellular carcinoma related to hepatitis B virus.