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Measurement of fibrosis in needle liver biopsies: Evaluation of a colorimetric method
Author(s) -
Jimenez Wladimiro,
Parés Albert,
Caballería Joan,
Heredia Daniel,
Bruguera Meguel,
Torres Miguel,
Rojkind Marcos,
Rodés Joan
Publication year - 1985
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840050517
Subject(s) - sirius red , cirrhosis , steatosis , fibrosis , pathology , chronic hepatitis , hepatic fibrosis , medicine , biopsy , liver biopsy , liver fibrosis , gastroenterology , immunology , virus
Collagen content was measured in 38 needle liver biopsies (8 steatosis, 8 chronic hepatitis, 7 fibrosis and 15 cirrhosis) by a new colorimetric method based on the selective capacity of Sirius red and Fast green to bind to collagen and noncollagenous proteins, respectively. The values were compared with those obtained after determination of the degree of fibrosis by morphometry in the same tissue. In biopsies with cirrhosis and fibrosis, there was a higher amount of collagen than in biopsies with chronic hepatitis and steatosis. Furthermore, there was a highly significant direct correlation between the collagen content measured colorimetrically and the degree of fibrosis determined morphometrically (r = 0.77, p < 0.001), suggesting that this new colorimetric method is useful in measuring the degree of fibrosis in needle liver biopsies.