Premium
Recovery of liver function in partially hepatectomized rats evaluated by aminopyrine demethylation capacity
Author(s) -
Sendama I.,
de Hemptinne B.,
Lambotte L.
Publication year - 1985
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840050418
Subject(s) - hepatectomy , medicine , liver function , demethylation , chemistry , portacaval shunt , liver regeneration , endocrinology , phenobarbital , liver function tests , cirrhosis , biology , biochemistry , surgery , regeneration (biology) , gene expression , resection , dna methylation , gene , microbiology and biotechnology , portal hypertension
Aminopyrine demethylation was investigated in rats after a 70% hepatectomy to assess possible parallelism between the recovery of mass and function. Tests were performed by analyzing 14 CO 2 exhalation from 0.1 μCi per 100 gm of body weight of [dimethylamine‐ 14 C]aminopyrine given intraperitoneally with incremental doses of unlabeled drug. Early after 70% hepatectomy, V max was reduced by 52%. This discordance between mass and function was not due to extrahepatic aminopyrine demethylation, since liver exclusion reduced demethylation of aminopyrine to nearly nil. Whether it results from increased liver blood flow in the remnant liver is less clear: portacaval anastomosis provoked an identical fall in V max and liver blood flow, but aortal‐portal fistula which increased liver blood flow by 70% did not modify V max , The early increase in V max could be related to a “hepatotrophic” factor of splanchnic origin which increased after partial hepatectomy and decreased after portacaval shunt. After the early period, V max , expressed per gram of actual liver weight, returned to control range. Throughout regeneration (4 to 144 hr), no modification was observed in K m nor in cytochrome P‐450 concentration. Enzymatic induction with phenobarbital increased the demethylation capacity more than liver weight in intact and regenerating liver. Except for the first hours after partial hepatectomy or after enzymatic induction, the aminopyrine demethylation capacity directly correlated with liver mass and may be useful in evaluating liver regeneration in vivo .