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A radioimmunoassay procedure for type III procollagen: Its use in the detection of hepatic fibrosis
Author(s) -
Galambos Michael R.,
Collins Delwood C.,
Galambos John T.
Publication year - 1985
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840050109
Subject(s) - radioimmunoassay , procollagen peptidase , biopsy , fibrosis , liver biopsy , medicine , hepatic fibrosis , gastroenterology , pathology
Recent studies suggest that the serum level of Type III procollagen (PC‐III) could be a valuable, noninvasive monitor of hepatic fibrogenesis. We have developed a sensitive and specific radioimmunoassay for PC‐III procollagen isolated and purified from fetal goat skin which shows high cross‐reaction for human PC‐III. In a double‐blind study, serum samples taken from 8 normal volunteers and 50 patients at the time of liver biopsy were assayed for PC‐III. Each biopsy was reviewed without knowledge of the radioimmunoassay results, and old or active fibrosis was graded on a 0 to 3 (none, minimal, moderate, severe) scale. There was a significant difference between serum concentrations of PC‐III in normals (mean ± S.D. = 72 ng per ml ± 8) as compared to patients with biopsy evidence of active fibrosis (mean ± S.D. = 153 ng per ml ± 12; p < 0.001). The serum concentration of PC‐III showed a good correlation (r = 0.58; p < 0.001) with the histological grade of active lobular fibrosis. Measurement of PC‐III is a noninvasive test for detection of active fibrosis in the liver.