Research Library

Premium Influence of Hydroxylation and Conjugation of Bile Salts on Their Membrane‐Damaging Properties‐Studies on Isolated Hepatocytes and Lipid Membrane Vesicles
Author(s)
SchÖLmerich Jürgen,
Becher MariaSybille,
Schmidt Karlheinz,
Schubert Rolf,
Kremer Bernd,
Feldhaus Susanne,
Gerok Wolfgang
Publication year1984
Publication title
hepatology
Resource typeJournals
PublisherW.B. Saunders
Abstract To characterize the relative toxicity of different bile salts, isolated hepatocytes were incubated with different concentrations of one bile salt or with identical concentrations of different bile salts and their conjugates. Incubation lasted for 1 hr; samples were taken at different intervals and studied for enzyme release, urea synthesis and stimulation by glucagon, and by electron microscopy. While the trihydroxylated bile salt, taurocholate, did not produce alterations at concentrations up to 1,500 μM , the dihydroxylated salts, chenodeoxy‐ and deoxycholate, caused enzyme release and membrane lysis, and inhibited urea synthesis at concentrations above 500 μM. In contrast, urso‐deoxycholate was ineffective at concentrations up to 1,500 μM. Conjugation of these bile salts did not result in significant differences with the exception of deoxycholate conjugates which induced enzyme leakage more rapidly. Studies of lipid membrane vesicles revealed corresponding alterations. The monohydroxylated salt, taurolithocholate, caused cellular damage as indicated by enzyme loss and impairment of hormonal sensitivity of cells at low concentrations (30 to 100 μM). Dihydroxylated salts produced a different time course of membrane leakage, ultrastructural changes and release of volume marker and lipid in liposomes, suggesting a possible different mechanism of damage induced by this bile salt. Both systems can readily be used to study bile salt membrane interactions.
Subject(s)biochemistry , chemistry , enzyme , hepatocyte , in vitro , lysis , membrane , urea , vesicle
Language(s)English
SCImago Journal Rank5.488
H-Index361
eISSN1527-3350
pISSN0270-9139
DOI10.1002/hep.1840040416

Seeing content that should not be on Zendy? Contact us.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here