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Demonstration of albumin receptors onisolated human hepatocytes by light and scanning electron microscopy
Author(s) -
Trevisan Alessandro,
Gudat Fred,
Guggenheim Richard,
Krey Gunthild,
Dürmüller Ursula,
Lüond Guido,
Düggelin Marcel,
Landmann Jonas,
Tondelli Pietro,
Bianchi Leonardo
Publication year - 1982
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840020616
Subject(s) - albumin , hepatocyte , human serum albumin , receptor , glutaraldehyde , chemistry , serum albumin , human albumin , scanning electron microscope , electron microscope , biophysics , biochemistry , microbiology and biotechnology , biology , chromatography , materials science , in vitro , physics , optics , composite material
The presence of albumin receptors on the plasma membrane of isolated human hepatocytes was investigated employing albumin‐coupled latex minibeads. Hepatocyte‐latex reaction was visualized by phase contrast and scanning electron microscopy. The experiments demonstrate that hepatocytes exhibit binding activity for polymeric and monomelic forms of glutaraldehyde‐treated albumin. Additionally, the reaction was shown to be species‐nonspecific. These findings support the hypothesis that polymerized albumin may act as a bridge between receptors on hepatitis B virus and human hepatocytes.