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Morphologic Study of Intermediate Filaments in Rat Hepatocytes
Author(s) -
French Samuel W.,
Kondo Isao,
Irie Tetsuya,
Ihrig Thomas J.,
Benson Nancy,
Munn Robert
Publication year - 2007
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840020106
Subject(s) - microtubule , cytoplasm , endoplasmic reticulum , vesicle , electron microscope , centriole , chemistry , biophysics , sodium dodecyl sulfate , intermediate filament , microbiology and biotechnology , cytoskeleton , polyacrylamide gel electrophoresis , biology , cell , biochemistry , membrane , physics , optics , enzyme
Rat livers perfused with 0.5% Triton X‐100 for 15 to 90 min followed by 1% sodium dodecyl sulfate for 30 min were studied by electron microscopy and polyaerylamide gel electrophoresis. After 15 min of perfusion, a rich network of intermediate filaments and microtubules was visualized in the cytoplasm of hepatocytes. Using stereopairs, branching was visualized. Connections of filaments were noted with nuclei, centrioles, microtubules, vesicles, and rough endoplasmic reticulum. The existence of connections supported the concept that intermediate filaments may function to integrate mechanically the cytoplasmic space as postulated by Lazarides.