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Hepatocyte and Kupffer cell functions during liver regeneration in streptozotocin‐diabetic rats
Author(s) -
Cornell Robert P.,
Hinck Brenda K.,
Halley Randall E.
Publication year - 1981
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840010510
Subject(s) - kupffer cell , phagocytosis , medicine , endocrinology , streptozotocin , hepatocyte , liver regeneration , regeneration (biology) , diabetes mellitus , insulin , hepatectomy , hypoglycemia , liver cytology , biology , immunology , surgery , biochemistry , microbiology and biotechnology , liver metabolism , resection , in vitro
Abstract The insulinoprivic influence of acute severe streptozotocin diabetes on liver regeneration in rats was evaluated by determining liver weights as well as hepatocyte and Kupffer cell functional capacities. Functional capacities were assessed by bromosulfophthalein uptake for hepatocytes and carbon phagocytosis for Kupffer cells. Evaluation immediately after partial hepatectomy revealed a 66% reduction of liver mass, a 63% decrease in hepatocyte bromosulfophthalein removal, and a 65% decline in Kupffer cell carbon phagocytosis. Per cent recovery at 48‐hr posthepatectomy was considerably greater for carbon phagocytosis than for bromosulfophthalein removal by regenerating livers. This apparent difference in functional recovery was likely due in part to enhanced non‐Kupffer cell carbon phagocytosis. No significant differences of the three regeneration indices were noted for untreated streptozotocin‐diabetic rats compared to nondiabetic animals. However, insulin administration to fasted streptozotocin diabetics significantly stimulated liver regeneration above that of untreated fasted rats and almost equivalent to that of pair‐fed animals. Fasted rats had in general slower liver regeneration than pair‐fed animals as expected. Furthermore, insulin administration to fasted nondiabetic rats after partial hepatectomy caused severe hypoglycemia and resulted in a further depression of liver regeneration.