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Is there a regeneration stimulator substance in the effluent from perfused partially hepatectomized livers?
Author(s) -
van HoornHickman Rosemary,
Kahn Del,
Green Jewel,
Macleod Heather A.,
Terblanche John
Publication year - 1981
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1002/hep.1840010402
Subject(s) - portacaval shunt , perfusion , liver regeneration , hepatectomy , portacaval anastomosis , medicine , fulminant hepatic failure , endocrinology , liver transplantation , regeneration (biology) , transplantation , biology , surgery , portal hypertension , cirrhosis , resection , microbiology and biotechnology
An attempt was made to transfer a stimulator substance from the perfusate of partially hepatectomized perfused livers to the portal stump of portacaval‐shunted pig recipients. Blood was either cross‐circulated with recipients during perfusion or was given by exchange tranfusion after 4 hr perfusion. There was an increase in thymidine kinase activity and mitotic indices in biopsies from portacaval‐shunted recipients whether perfusions were performed 2 or 4 days after partial hepatectomy. Blood perfused through intact livers did not enhance the regenerative response of portacaval‐shunted pigs; in fact it appeared to suppress the normal small response. Approximately 10% of the portacaval‐shunted recipients died acutely when perfusate was infused into the portal stump. This did not occur if saline or unperfused blood was used suggesting that some toxic substance accumulated during liver perfusion. In end‐stage fulminant hepatic failure, use of blood perfused through a partially hepatectomized animal liver may provide the regenerative stimulus which often appears to be lacking.