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Epidermal growth factor receptor gene amplification and expression in head and neck cancer cell lines
Author(s) -
Weichselbaum Ralph R.,
Dunphy Edward J.,
Beckett Michael A.,
Tybor Allen G.,
Moran William J.,
Goldman Michael E.,
Vokes Everett E.,
Panje William R.
Publication year - 1989
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.2880110510
Subject(s) - epidermal growth factor receptor , cell culture , epidermal growth factor , cancer research , biology , head and neck squamous cell carcinoma , cell growth , cell , head and neck , a431 cells , gene expression , receptor , growth factor receptor , carcinoma , gene , cancer , pathology , head and neck cancer , medicine , cell cycle , genetics , surgery , molecular medicine
We studied epidermal growth factor receptor (EGFR) gene am plification and expression in 11 early passage human head and neck carcinoma cell lines. Three cell lines demonstrated EGFR gene amplification and 10 lines showed an increase in EGFR mRNA when compared with normal keratinocytes, placenta, and a human skin carcinoma cell line. The effects of EGF on growth in 6 head and neck carcinoma cell lines was also studied. Growth inhibition at a concentration of 20 ng/mL was observed in one cell line but had no effect on growth in 5 cell lines. An increase in EGFR may be important in the etiology of, or progression of, head and neck carcinoma although the mecha nisms need to be elucidated by further study.