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Characteristic differences in radiation‐induced DNA damage response in human papillomavirus‐negative and human papillomavirus‐positive head and neck cancers with accumulation of fractional radiation dose
Author(s) -
Reid Paul,
Staudacher Alexander H.,
Marcu Loredana G.,
Olver Ian,
Moghaddasi Leyla,
Brown Michael P.,
Bezak Eva
Publication year - 2021
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.26802
Subject(s) - head and neck squamous cell carcinoma , micronucleus test , cancer research , dna damage , biomarker , radiation therapy , medicine , oncology , cell culture , cell , human papillomavirus , head and neck cancer , biology , dna , toxicity , genetics
Abstract Background Superior treatment responses by patients with human papillomavirus (HPV) positive head and neck squamous cell carcinoma (HNSCC), compared to patients with HNSCC from other causes, drive biomarker research to optimize treatment. Most HNSCC patients receive radiation therapy delivered as a fractionated course. Changing HPV status in HNSCC from a positive prognostic marker to a predictive one requires biomarkers that capture cellular radiation response to cumulative dose. Methods Nuclear enlargement, γH2AX expression and micronuclei count, were studied in six HNSCC cell lines after 4 Gy fractionated X‐irradiation. Results All HNSCC cell lines displayed altered cellular responses, indicating increasing inability to repair radiation damage with subsequent radiation fractions. Increases in nuclear area were significantly greater among HPV positive cell lines (207% and 67% for the HPV positive and HPV negative groups, respectively). Conclusions A different character of DNA repair dysfunction in the HPV positive group suggests greater chromosomal translocation with accumulated radiation dose.