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Clinical validation of 13‐gene DNA methylation analysis in oral brushing samples for detection of oral carcinoma: Italian multicenter study
Author(s) -
Gissi Davide B.,
Morandi Luca,
Colella Giuseppe,
De Luca Roberto,
Campisi Giuseppina,
Mauceri Rodolfo,
Romeo Umberto,
Tenore Gianluca,
Mignogna Michele D.,
Adamo Daniela,
Oteri Giacomo,
Marcianò Antonia,
Vescovi Paolo,
Meleti Marco,
Serpico Rosario,
Di Stasio Dario,
Pentenero Monica,
Santarelli Andrea,
Bianchi Alberto,
Crimi Salvatore,
Marchetti Claudio,
Tarsitano Achille,
Foschini Maria Pia,
Montebugnoli Lucio
Publication year - 2021
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.26624
Subject(s) - medicine , basal cell , dna methylation , gastroenterology , pathology , oncology , gene , biology , gene expression , genetics
Background The aim of this Italian multicenter study was to evaluate the diagnostic performance of a minimally invasive method for the detection of oral squamous cell carcinoma (OSCC) based on 13‐gene DNA methylation analysis in oral brushing samples. Methods Oral brushing specimens were collected in 11 oral medicine centers across Italy. Twenty brushing specimens were collected by each center, 10 from patients with OSCC, and 10 from healthy volunteers. DNA methylation analysis was performed in blindness, and each sample was determined as positive or negative based on a predefined cutoff value. Results DNA amplification failed in 4 of 220 (1.8%) samples. Of the specimens derived from patients with OSCC, 93.6% (103/110) were detected as positive, and 84.9% (90/106) of the samples from healthy volunteers were negative. Conclusion These data confirmed the diagnostic performance of our novel procedure in a large cohort of brushing specimens collected from 11 different centers and analyzed in blindness.