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Synergistic effects of combined treatment with ultrasound‐mediated cisplatin‐loaded microbubbles and atorvastatin on head and neck cancer
Author(s) -
Liao AiHo,
Lin WanTing,
Chen HangKang,
Shih ChengPing,
Wang ChihHung,
Chu YuengHsiang
Publication year - 2021
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.26445
Subject(s) - atorvastatin , cisplatin , microbubbles , viability assay , glutathione , chemistry , apoptosis , pharmacology , ultrasound , cytotoxicity , medicine , cancer research , chemotherapy , in vitro , biochemistry , enzyme , radiology
Background Previously, we used ultrasound (US)‐mediated cisplatin (CDDP)‐loaded microbubbles (CDDP‐MBs) to increase intratumoral CDDP level while decreasing systemic cytotoxicity. Statins have shown antitumorigenic properties. Our study investigated the effects of atorvastatin with CDDP‐MBs and US on head neck cancer. Methods Cell viability analysis with CDDP‐MBs and atorvastatin combined with US in FaDu cell line were tested. Cell proliferation and glutathione level were also evaluated. Results Both CDDP and atorvastatin reduced cell's viability. Coadministration of CDDP and atorvastatin resulted in synergistic inhibitory effect. After US sonication, cell viability with atorvastatin and CDDP was significantly reduced for CDDP combined with MBs (65.98% to 49.13%) and for CDDP‐MBs (86.17% to 50.15%). CDDP‐MBs combined with atorvastatin and US inhibited the proliferation of cells: 19.61% for CDDP‐MBs + atorvastatin + US, 36.28% for CDDP + atorvastatin, and 71.73% for atorvastatin alone. Also, CDDP‐MBs + atorvastatin + US induced apoptosis by decreasing cellular level of glutathione. Conclusions Atorvastatin combined with MB‐conjugated CDDP exerts synergistic inhibitory effect on head neck cancer.