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Inhibition of tropomyosine receptor kinase B on the migration of human Schwann cell and dispersion of oral tongue squamous cell carcinoma in vitro
Author(s) -
Ein Liliana,
Bracho Olena,
Mei Christine,
Patel Jaimin,
Boyle Thomas,
Monje Paula,
FernandezValle Cristina,
Bas Esperanza,
Thomas Giovana,
Weed Donald,
Sargi Zoukaa,
Dinh Christine
Publication year - 2019
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.25956
Subject(s) - tropomyosin receptor kinase b , cell migration , neurotrophic factors , cancer cell , cell , cancer research , cancer , chemistry , microbiology and biotechnology , biology , receptor , medicine , biochemistry
Background Schwann cells (SC) may play an important role in perineural invasion (PNI) by promoting cancer cell dispersion. Brain‐derived neurotrophic factor (BDNF) may contribute to these cellular events by activating tropomyosine receptor kinase B (TrkB). This study examines the effect of TrkB inhibition on SC migration and oral cancer cell dispersion in vitro. Methods Human tongue squamous cell carcinoma (SCC‐9) and human SCs were cocultured in three different conditioned mediums: control, BDNF, and TrkB inhibitor. Cell migration, cancer cell dispersion, and SC dedifferentiation were measured on time‐lapse and immunofluorescence images. Results Cancer cell migration exceeded SC migration in all conditions. TrkB inhibition promoted SC dedifferentiation and significantly increased SC migration, when compared to BDNF conditions. TrkB inhibition also reduced cancer cell dispersion, when compared to control and BDNF‐treated cultures. Conclusion SCs may have importance in the pathophysiology of PNI. TrkB inhibition may be a potential avenue for therapeutic intervention.