Premium
Young and elderly oral squamous cell carcinoma patients present similar angiogenic profile and predominance of M2 macrophages: Comparative immunohistochemical study
Author(s) -
Teixeira Lucas R.,
Almeida Luciana Y.,
Silva Rodrigo N.,
Mesquita Ana T. M.,
Colturato Carla B. N.,
Silveira Heitor A.,
Duarte Andressa,
RibeiroSilva Alfredo,
León Jorge E.
Publication year - 2019
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.25954
Subject(s) - cd163 , cd68 , immunohistochemistry , angiogenesis , cd34 , immunosenescence , neovascularization , macrophage , medicine , basal cell , pathology , microvessel , cancer , cancer research , immunology , biology , immune system , stem cell , in vitro , biochemistry , genetics
Background M2 macrophages are often detected in oral squamous cell carcinoma (OSCC), which, influenced by hypoxic conditions, appear to have high angiogenesis‐inducing capacity. However, the effects of immunosenescence on tumor‐associated macrophages (TAMs) and angiogenesis in OSCC are unknown. Methods Fifty‐seven OSCCs were divided into 3 groups (I: <40 years [n = 17]; II: 40‐65 years [n = 20]; III: >65 years [n = 20]). Immunohistochemistry for CD68 and CD163 (TAMs), and CD34 and D2‐40 for microvessel density (MVD), microvessel area (MVA), and total vascular area (TVA) were performed. Results All groups showed similar clinicopathological and immunohistochemical findings. Similar CD68 and CD163 expression, confirmed a M2 phenotype. MVD, MVA, and TVA were similar, however, with significant predominance of blood vessels. No significant correlation between macrophage and angiogenic markers was observed. Conclusions A similar TAM and angiogenesis profile suggests the participation of other mechanisms, instead immunosenescence, in young and elderly OSCC patients.