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Heterozygosity of p16 expression in an oral squamous cell carcinoma with associated loss of heterozygosity and copy number alterations
Author(s) -
Ambele Melvin A.,
Pepper Michael S.,
Heerden Marlene B.,
Heerden Willie F. P.
Publication year - 2019
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.25566
Subject(s) - field cancerization , loss of heterozygosity , biology , pathology , cancer , basal cell , cancer research , gene , medicine , genetics , allele
Background Oral field cancerization describes a multifocal development process involving many cells at once in response to prolong exposure to carcinogens. This case demonstrated differential p16 expression in different sections of the same HPV negative tumor on the floor of mouth in a patient with history of prolonged smoking. Methods Histological examination, presence of HPV infection and OncoScan analysis of DNA extracted from two well‐defined areas with different p16 expression profiles were performed. Results Histological and immunochemical analysis revealed the presence of a dual architectural pattern squamous cell carcinoma with a p16 negative and a p16 positive component. OncoScan analysis showed genetic changes that define field cancerization of the p16 negative tumor as revealed by mosaicism in both loss of heterozygosity and copy number alterations in cancer‐associated genes located on 3p, 7p, 9p 11q, and 17p. Conclusion These changes were indicative of field cancerization in response to tobacco exposure.