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Promotion of skin wound healing using prevascularized oral mucosal cell sheet
Author(s) -
Lee Jaewang,
Shin Daiha,
Roh JongLyel
Publication year - 2019
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.25432
Subject(s) - in vivo , wound healing , fibrin , progenitor cell , cell , medicine , stem cell , immunology , chemistry , microbiology and biotechnology , biology , biochemistry
Background This study examined the potential use of our newly developed prevascularized oral mucosal cell sheet for the treatment of skin wounds. Methods Mucosal cell sheets containing cultured keratinocytes and plasma fibrin without (K sheet) or with a mixture of fibroblasts and endothelial progenitor cells (PV sheet) were transplanted into full‐thickness skin excisional wounds of nude mice. Results This technique was successful for in vitro culture; expanding keratinocytes, fibroblasts, and endothelial progenitor cells; and generating prevascularized mucosal cell sheets. Cell sheets promoted in vivo wound healing with rapid wound closure and less scarring compared to controls. This result was more apparent in the PV than the K sheet ( P < .05). Wounds covered with cell sheets showed less expression of TGFB1 , ACTA2 , and FN1 mRNAs than the controls ( P < .05). Conclusion The prevascularized mucosal cell sheet showed in vivo efficacy and tissue plasticity in cutaneous wounds by promoting accelerated healing.