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Differential expression and prognostic value of long non‐coding RNA in HPV‐negative head and neck squamous cell carcinoma
Author(s) -
Haque SulsalUl,
Niu Liang,
Kuhnell Damaris,
Hendershot Jacob,
Biesiada Jacek,
Niu Wen,
Hagan Matthew C.,
Kelsey Karl T.,
Casper Keith A.,
WiseDraper Trisha M.,
Medvedovic Mario,
Langevin Scott M.
Publication year - 2018
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.25136
Subject(s) - head and neck squamous cell carcinoma , meg3 , long non coding rna , biology , cancer research , oncology , rna , head and neck cancer , cancer , medicine , gene , genetics
Abstract Background Long non‐coding RNA (lncRNA) has emerged as a new avenue of interest due to its various biological functions in cancer. Abnormal expression of lncRNA has been reported in other malignancies but has been understudied in head and neck squamous cell carcinoma (HNSCC). Methods The lncRNA expression was interrogated via quantitative real‐time polymerase chain reaction (qRT‐PCR) array for 19 human papillomavirus (HPV)‐negative HNSCC tumor‐normal pairs. The Cancer Genome Atlas (TCGA) was used to validate these results. The association between differentially expressed lncRNA and survival outcomes was analyzed. Results Differential expression was validated for 5 lncRNA ( SPRY4‐IT1 , HEIH , LUCAT1 , LINC00152 , and HAND2‐AS1 ). There was also an inverse association between MEG3 expression (not significantly differentially expressed in TCGA tumors but highly variable expression) and 3‐year recurrence‐free survival (RFS). Conclusion We identified and validated differential expression of 5 lncRNA in HPV‐negative HNSCC. Low MEG3 expression was associated with favorable 3‐year RFS, although the significance of this finding remains unclear.