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Targeting of miR‐31/96/182 to the Numb gene during head and neck oncogenesis
Author(s) -
Chou ChungHsien,
Tu HsiFeng,
Kao ShouYen,
Chiang ChunYu Fan,
Liu ChungJi,
Chang KuoWei,
Lin ShuChun
Publication year - 2018
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.25063
Subject(s) - numb , microrna , downregulation and upregulation , carcinogenesis , gene knockdown , head and neck squamous cell carcinoma , cancer research , biology , untranslated region , three prime untranslated region , gene , messenger rna , cancer , head and neck cancer , microbiology and biotechnology , genetics
Background MicroRNAs (miRNAs) play crucial roles in head and neck squamous cell carcinoma (HNSCC). This study investigates whether miR‐31, miR‐96 , and miR‐182 are involved in targeting Numb during HNSCC. Methods The expression of miR‐31/96/182 in tumor tissues was analyzed. Reporter assay, knockdown, expression, and oncogenic analysis were carried out in cell lines. Results Upregulation of miR‐31/96/182 was detected in tumor tissues. In addition, advanced tumors showed higher expression levels of these miRNAs. The expression of these miRNAs was upregulated after treatment with areca ingredients ( P < .01 or P < .001). These miRNAs directly targeted the 3′ untranslated region (UTR) sequence of the Numb gene. An increased migration and invasion of HNSCC cells was associated with the exogenous expression of miR‐31/96/182 ( P < .01 or P < .001), and this was reverted by expression of Numb . Conclusion This study provides new evidence demonstrating that there is frequent and concordant upregulation of miR‐31, miR‐96 , and miR‐182 during HNSCC and these miRNAs co‐target Numb .