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Preventive effects of tonsil‐derived mesenchymal stem cells on osteoradionecrosis in a rat model
Author(s) -
Park Hae Sang,
Lee Jihae,
Kim JinWoo,
Kim Ha Young,
Jung Soo Yeon,
Lee Sung Min,
Park Chan Hum,
Kim Han Su
Publication year - 2018
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.25004
Subject(s) - tonsil , osteoradionecrosis , matrigel , mesenchymal stem cell , medicine , dentistry , extraction (chemistry) , surgery , pathology , chemistry , radiation therapy , angiogenesis , chromatography
Background The purpose of this study was to investigate the effects of tonsil‐derived mesenchymal stem cells (MSCs) on osteoradionecrosis (ORN). Method We generated a mandibular ORN rat model using a combination of 20‐Gy single‐dose irradiation and tooth extraction. Study groups were negative control (tooth extraction only), ORN group (irradiation, tooth extraction), Matrigel‐1 group (Matrigel; BD Biosciences, San Jose, CA; irradiation, Matrigel application immediately after tooth extraction), tonsil‐derived MSC‐1 group (irradiation, tonsil‐derived MSC application immediately after tooth extraction), Matrigel‐4 group (irradiation, Matrigel application 4 weeks after tooth extraction), and tonsil‐derived MSC‐4 group (irradiation, tonsil‐derived MSC application 4 weeks after tooth extraction). Result Bone mineral density was significantly lower in the ORN group than in the negative control group. The tonsil‐derived MSC‐1 group showed significantly higher bone mineral density than did the ORN and tonsil‐derived MSC‐4 groups. Conclusion A single 20‐Gy dose of irradiation combined with tooth extraction successfully generated ORN in the rat model. The tonsil‐derived MSCs can be effective for bone regeneration in ORN, particularly when applied immediately after dentoalveolar trauma or surgery.