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Radioprotective effect of thymoquinone on salivary gland of rats exposed to total cranial irradiation
Author(s) -
Akyuz Mehmet,
Taysi Seyithan,
Baysal Elif,
Demir Elif,
Alkis Hilal,
Akan Muslum,
Binici Habip,
Karatas Zeynel Abidin
Publication year - 2017
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.24861
Subject(s) - thymoquinone , superoxide dismutase , chemistry , xanthine oxidase , malondialdehyde , glutathione peroxidase , free radical scavenger , antioxidant , glutathione , nitric oxide , pharmacology , biochemistry , oxidative stress , intraperitoneal injection , scavenger , enzyme , medicine , organic chemistry
Background The purpose of this study was to investigate the radioprotective effects of thymoquinone against radiation‐induced damage in the salivary glands of rats exposed to total cranial gamma irradiation. Methods Thirty‐two Sprague‐Dawley rats were divided into 4 groups to test the radioprotective effectiveness of thymoquinone by intraperitoneal injection. An appropriate control group was also studied. Biochemical parameters in liver tissue of rats were determined by spectrophotometer. Results Glutathione peroxidase (GSH‐Px), glutathione‐S‐transferase (GST), total (enzymatic plus nonenzymatic) superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), and superoxide dismutase (SOD) activities were significantly decreased, whereas xanthine oxidase, nitric oxide synthase activities, malondialdehyde, nitric oxide, and peroxynitrite levels were significantly increased in the irradiation group when compared to the control and sham control groups. Conclusion Results showed that thymoquinone reduces oxidative and nitrosative stress parameters and has antioxidant effects and a free radical scavenging activity.