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Vascular endothelial growth factor–targeted paclitaxel‐loaded liposome microbubbles and inhibition of human epidermoid‐2 cell proliferation
Author(s) -
Zhou Lian,
Luo Wenlong
Publication year - 2017
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.24648
Subject(s) - flow cytometry , microbubbles , vascular endothelial growth factor , cell growth , cell cycle , blot , apoptosis , cell , paclitaxel , mtt assay , cancer research , chemistry , bromodeoxyuridine , microbiology and biotechnology , pathology , biology , medicine , cancer , vegf receptors , ultrasound , biochemistry , gene , radiology
ABSTRACT Background Vascular endothelial growth factor (VEGF) plays an important role in the formation of capillary blood vessels. The purpose of this study was to evaluate the inhibitory effect of VEGF‐targeted paclitaxel (PTX)‐loaded liposome microbubbles (VTPLLMs) on the proliferation of human epidermoid (Hep‐2) laryngeal squamous cell carcinoma (SCC). Methods Six groups were randomly divided. The inhibitory effects on Hep‐2 proliferation were assessed by a methylthiazol tetrazolium (MTT) method. The cell cycle distributions were investigated by flow cytometry. The expression variations of VEGF and caspase‐3 were compared by Western blotting. Results Proliferation of Hep‐2 in the VTPLLM+ultrasound group was significantly inhibited, and flow cytometry showed that the Hep‐2 cells were significantly blocked at stage G2/M compared with other groups. Moreover, Western blotting showed VEGF expression was downregulated and caspase‐3 expression was upregulated. Conclusion VTPLLMs can efficiently inhibit tumor cell proliferation and outperform nontargeted microbubbles or PTX. © 2017 Wiley Periodicals, Inc. Head Neck 39: 656–661, 2017