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Surgical margins in head and neck squamous cell carcinoma: Effect of heat artifact on immunohistochemistry as a future tool for assessment
Author(s) -
Sigston Elizabeth A. W.,
Longano Anthony,
Strzelecki Aneta T.,
Williams Bryan R.G.
Publication year - 2016
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.24450
Subject(s) - immunohistochemistry , h&e stain , pathology , head and neck squamous cell carcinoma , staining , medicine , cancer , head and neck , carcinoma , head and neck cancer , surgery
Background Margins in head and neck squamous cell carcinoma (HNSCC) are determined by morphological changes assessed via hematoxylin‐eosin staining. Physiological changes may not be detected by this technique. The purpose of this study was to determine if a protein biomarker, laminin‐332γ2, overexpressed in cancer cells at the invasive front in HNSCC, remains unaffected by heat produced during resection, supporting a role for immunohistochemistry assessment of margins. Methods Archived tissue blocks from glottic squamous cell carcinomas (SCCs) resected by CO2 laser likely to contain both cancer cells and artifact were identified; 129‐paired slides were obtained. One slide of each pair was stained with hematoxylin‐eosin; the second stained for laminin‐332γ2. The presence of cancer cells, artifact, and positive laminin‐332γ2 staining was recorded. Twenty‐seven pairs met the inclusion criteria. Results Immunohistochemistry staining of laminin‐332γ is preserved in presence of heat artifact. Conclusion This study supports use of immunohistochemistry to assess margins. © 2016 Wiley Periodicals, Inc. Head Neck 38: 1401–1406, 2016