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Human olfactory stem cells for injured facial nerve reconstruction in a rat model
Author(s) -
Batioglu–Karaaltin Aysegul,
Karaaltin Mehmet Veli,
Oztel Olga Nehir,
Ovali Ercument,
Sener Belit Merve,
Adatepe Turgut,
Yigit Ozgur,
Bozkurt Erol,
Baydar Serap Yesilkir,
Bagirova Melahat,
Uzun Nurten,
Allahverdiyev Adil
Publication year - 2016
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.24371
Subject(s) - stem cell , olfactory mucosa , facial nerve , anatomy , saline , olfactory system , medicine , biology , neuroscience , microbiology and biotechnology
Abstract Background The purpose of this study was to show the efficacy of olfactory stem cells for injured facial nerve reconstruction in a rat model. Methods Olfactory stem cells were isolated from the olfactory mucosa of human participants. A 2‐mm excision was performed on the right facial nerve of all rats. Reconstruction was performed with a conduit in group 1 ( n = 9); a conduit and phosphate‐buffered saline in group 2 ( n = 9); and a conduit and labeled olfactory stem cell in group 3 ( n = 9). Rats were followed for whisker movements and electroneuronography (ENoG) analyses. Results The whisker‐movement scores for group 3 were significantly different from other groups ( p < .001). ENoG showed that the amplitude values for group 3 were significantly different from group 1 and group 2 ( p = .030; p < .001). Group 3 showed marked olfactory stem cell under a fluorescence microscope. Conclusion This study suggests that olfactory stem cells may be used as a potent cellular therapy for accelerating the regeneration of peripheral nerve injuries. © 2016 Wiley Periodicals, Inc. Head Neck 38 : E2011–E2020, 2016