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Decreased mitochondrial copy numbers in oral squamous cell carcinoma
Author(s) -
Takeda Daisuke,
Hasegawa Takumi,
Ueha Takeshi,
Sakakibara Akiko,
Kawamoto Teruya,
Minamikawa Tsutomu,
Sakai Yoshitada,
Komori Takahide
Publication year - 2016
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.24194
Subject(s) - tfam , mitochondrial dna , biology , mitochondrion , immunohistochemistry , taqman , coactivator , microbiology and biotechnology , real time polymerase chain reaction , cancer , carcinoma , cell , pathology , cancer research , transcription factor , gene , medicine , genetics , immunology
Background Mitochondrial dysfunction and altered respiration have long been suspected to affect the development and progression of cancer. Although quantitative changes in mitochondrial DNA (mtDNA) have been reported in head and neck squamous cell carcinoma (SCC), differences in mtDNA copy numbers between normal and cancerous tissues from same patients have not been assessed. Methods We compared mtDNA copy numbers and expressions of peroxisome proliferator‐activated receptor gamma coactivator‐1 alpha (PGC‐1α) and mitochondrial transcription factor A (TFAM) between normal mucous membrane and cancerous tissues resected from 35 patients with oral SCC, using TaqMan quantitative real‐time polymerase chain reaction (PCR) and immunohistochemical staining. Results We found mtDNA copy numbers and expressions of PGC‐1α and TFAM were decreased in cancerous tissues compared with normal tissues from the same patients. Conclusion The PGC‐1α–TFAM mitochondrial pathway may be associated with malignant potential in human oral SCC, and could be an attractive therapeutic target. © 2016 Wiley Periodicals, Inc. Head Neck 38:1170–1175, 2016