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Evaluation of individual sensitivity of head and neck squamous cell carcinoma to cetuximab by short‐term culture of tumor slices
Author(s) -
Peria Marine,
Donnadieu Jérôme,
Racz Caroline,
Ikoli JeanFortuné,
Galmiche Antoine,
Chauffert Bruno,
Page Cyril
Publication year - 2016
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.24126
Subject(s) - cetuximab , head and neck squamous cell carcinoma , medicine , head and neck cancer , immunohistochemistry , oncology , cancer , cancer research , cell , colorectal cancer , pathology , biology , genetics
Background Cetuximab is a targeted therapy with demonstrated efficacy in the management of head and neck squamous cell carcinoma (HNSCC). However, no laboratory assay is available to predict its efficacy in an individual patient. Methods Short‐term cultures of tumor slices were performed on 9 tumor samples obtained after surgical resection in patients. Cancer cell proliferation was evaluated by immunohistochemistry and the impact of cetuximab on cell proliferation was examined. Results Tumor architecture and the heterogeneous composition of HNSCC were preserved for at least 48 hours during short‐term culture of tumor slices. HNSCC cells demonstrated a heterogeneous individual response to cetuximab. Conclusion Short‐term culture of tumor slices is a strategy to estimate the clinical activity of cetuximab in individual patients with HNSCC. Further studies are required to correlate the results obtained with the clinical response of individual patients to cetuximab. © 2015 Wiley Periodicals, Inc. Head Neck 38 : E911–E915, 2016