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Establishment of a novel human papillomavirus–negative and radiosensitive head and neck squamous cell carcinoma cell line
Author(s) -
Kim KyungMin,
Park EunJi,
Yeo Jiyoung,
Joo YoungHoon,
Cho KwangJae,
Kim MinSik
Publication year - 2016
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.24037
Subject(s) - immunostaining , cell culture , head and neck squamous cell carcinoma , head and neck cancer , cancer research , cell , pathology , medicine , cancer , immunohistochemistry , biology , genetics
Background The purpose of this study was to develop a CMCSCC−1 cell line for head and neck cancer research into new therapies for head and neck squamous cell carcinoma (HNSCC). Methods The CMCSCC‐1 cell line was isolated from a primary oral tongue tumor specimen of a female patient. Tumor cells were evaluated for biomarkers expression by Western blots, reverse transcriptase‐polymerase chain reaction (RT‐PCR), fluorescence activated cell sorter, and immunostaining. Cell proliferation in response to radiation was measured by the WST‐8 assay. Results The characterization analyses revealed a typical epithelial morphology; a doubling time of approximately 24 hours, high tumorigenicity in immunodeficient mice, and upregulated biomarkers. CMCSCC‐1 cells were negative for human papillomavirus (HPV) infection, but more sensitive to radiation compared with those FaDu cell lines. Conclusion CMCSCC‐1, a novel oral tongue SCC cell line, was established. It will help in the elucidation of the molecular pathogenesis of HPV‐negative radiosensitive tumors. © 2015 Wiley Periodicals, Inc. Head Neck 38: E542–E551, 2015