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Novel method of cell line establishment utilizing fluorescence‐activated cell sorting resulting in 6 new head and neck squamous cell carcinoma lines
Author(s) -
Owen John Henry,
Graham Martin P.,
Chinn Steven B.,
Darr Owen F.,
Chepeha Douglas B.,
Wolf Gregory T.,
Bradford Carol R.,
Carey Thomas E.,
Prince Mark E.P.
Publication year - 2016
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.24019
Subject(s) - cell culture , cell sorting , head and neck squamous cell carcinoma , cell , fibroblast , population , cancer research , cancer , pathology , head and neck cancer , head and neck , biology , medicine , genetics , surgery , environmental health
Background The purpose of this study was to present the establishment of new cell lines, which is important to cancer research. Methods Six new head and neck squamous cell carcinoma cell lines were established using a novel fluorescence‐activated cell sorting (FACS) method in order to overcome the barrier of fibroblast overgrowth and the susceptibility of primary tumors to fail in vitro. Results Antibodies chosen for specific targeting of epithelial cells and fibroblasts successfully separated cells for line establishment in 6 of 12 attempts, providing an alternative method of establishing head and neck squamous cell carcinoma cell lines. Each attempt at cell line establishment resulted in an epithelial carcinoma population, which was genotyped and catalogued as a unique cell line, and a corresponding fibroblast population. Conclusion The selection of antibody markers could be optimized to aid in the establishment of any cancer cell line derived from any tumor tissue; this method is not limited to head and neck cancer. © 2015 Wiley Periodicals, Inc. Head Neck 38 : E459–E467, 2016