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Near‐infrared fluorescence imaging‐guided surgery improves recurrence‐free survival rate in novel orthotopic animal model of head and neck squamous cell carcinoma
Author(s) -
Atallah Ihab,
Milet Clément,
Henry Maxime,
Josserand Véronique,
Reyt Emile,
Coll JeanLuc,
Hurbin Amandine,
Righini Christian Adrien
Publication year - 2016
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.23980
Subject(s) - medicine , head and neck squamous cell carcinoma , head and neck cancer , animal model , fluorescence lifetime imaging microscopy , head and neck , resection , basal cell , optical imaging , cancer , pathology , fluorescence , radiology , surgery , physics , quantum mechanics , optics
Background Appropriate animal models are required to test novel therapeutics for head and neck squamous cell carcinoma (HNSCC) such as near‐infrared (NIR) imaging‐guided surgery. Methods We developed an optimized animal model of orthotopic HNSCC (in female athymic NMRI (Naval Medical Research Institute) nude mice) with a prolonged survival time. Resection of the orthotopic tumors was performed 30 days after implantation with or without the aid of a miniaturized clinical grade NIR optical imaging device, after systemic administration of a fluorescent RGD‐based probe that targets α v β 3 integrin. Results NIR optical imaging‐guided surgery increased the recurrence‐free survival rate by 50% through the detection of fluorescent cancer residues as small as 185 µm; these fragments could remain unidentified if resection was performed exclusively under unaided visual guidance. Conclusion NIR optical imaging‐guided surgery showed an improved HNSCC tumor resection quality in our optimized orthotopic animal model. © 2015 Wiley Periodicals, Inc. Head Neck 38 : E246–E255, 2016