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Computer‐aided molecular pathology interpretation in exploring prospective markers for oral submucous fibrosis progression
Author(s) -
Anura Anji,
Conjeti Sailesh,
Das Raunak Kumar,
Pal Mousumi,
Paul Ranjan Rashmi,
Bag Swarnendu,
Ray Ajoy Kumar,
Chatterjee Jyotirmoy
Publication year - 2016
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.23962
Subject(s) - oral submucous fibrosis , immunohistochemistry , pathology , medicine , stain , vascular endothelial growth factor , fibrosis , neovascularization , stromal cell , angiogenesis , staining , cancer research , vegf receptors
Background Evaluation of molecular pathology markers using a computer‐aided quantitative assessment framework would help to assess the altered states of cellular proliferation, hypoxia, and neoangiogenesis in oral submucous fibrosis and could improve diagnostic interpretation in gauging its malignant potentiality. Methods Immunohistochemical (IHC) expression of c‐Myc, hypoxia‐inducible factor‐1‐alpha (HIF‐1α), vascular endothelial growth factor (VEGF), VEGFRII, and CD105 were evaluated in 58 biopsies of oral submucous fibrosis using computer‐aided quantification. After digital stain separation of original chromogenic IHC images, quantification of the diaminobenzidine (DAB) reaction pattern was performed based on intensity and extent of cytoplasmic, nuclear, and stromal expression. Results Assessment of molecular expression proposed that c‐Myc and HIF‐1α may be used as strong screening markers, VEGF for risk‐stratification and VEGFRII and CD105 for prognosis of precancer into oral cancer. Conclusion Our analysis indicated that the proposed method can help in establishing IHC as an effective quantitative immunoassay for molecular pathology and alleviate diagnostic ambiguities in the clinical decision process. © 2015 Wiley Periodicals, Inc. Head Neck 38: 653–669, 2016