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Silk‐elastin‐like protein polymer matrix for intraoperative delivery of an oncolytic vaccinia virus
Author(s) -
Price Daniel L.,
Li Pingdong,
Chen ChunHao,
Wong Danni,
Yu Zhenkun,
Chen Nanhai G.,
Yu Yong A.,
Szalay Aladar A.,
Cappello Joseph,
Fong Yuman,
Wong Richard J.
Publication year - 2016
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.23877
Subject(s) - oncolytic virus , vaccinia , virology , virus , elastin , viral matrix protein , medicine , biology , recombinant dna , pathology , gene , genetics
Background Oncolytic viral efficacy may be limited by the penetration of the virus into tumors. This may be enhanced by intraoperative application of virus immediately after surgical resection. Methods Oncolytic vaccinia virus GLV‐1h68 was delivered in silk‐elastin‐like protein polymer (SELP) in vitro and in vivo in anaplastic thyroid carcinoma cell line 8505c in nude mice. Results GLV‐1h68 in SELP infected and lysed anaplastic thyroid cancer cells in vitro equally as effectively as in phosphate‐buffered saline (PBS), and at 1 week retains a thousand fold greater infectious plaque‐forming units. In surgical resection models of residual tumor, GLV‐1h68 in SELP improves tumor control and shows increased viral β‐galactosidase expression as compared to PBS. Conclusion The use of SELP matrix for intraoperative oncolytic viral delivery protects infectious viral particles from degradation, facilitates sustained viral delivery and transgene expression, and improves tumor control. Such optimization of methods of oncolytic viral delivery may enhance therapeutic outcomes. © 2014 Wiley Periodicals, Inc. Head Neck 38: 237–246, 2016