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Pilot study: Raman spectroscopy in differentiating premalignant and malignant oral lesions from normal mucosa and benign lesions in humans
Author(s) -
Guze Kevin,
Pawluk Hanna C.,
Short Michael,
Zeng Haishan,
Lorch Jochen,
Norris Charles,
Sonis Stephen
Publication year - 2015
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.23629
Subject(s) - pathology , lesion , raman spectroscopy , medicine , oral mucosa , optics , physics
Background Current practice for differentiating tissue lesions are based on histopathological criteria. This process is subject to error. The purpose of this study was to test whether an alternative, tissue‐based molecular signatures Raman spectra could be used to differentiate premalignant and malignant lesions from normal mucosa or benign lesions. Methods Eighteen treatment naive subjects with histologically diagnosed oral disease were studied by comparing the Raman spectra of lesions with contralateral healthy sites. Principle component and multivariate analysis were used to predict which of the tissue groups the average spectrum of each lesion or normal tissue belonged. Results The average spectra were clearly different between premalignant and malignant lesions and those derived from normal, benign tissues. Premalignant and malignant lesions could be predicted with 100% sensitivity and 77% specificity. Conclusion Raman spectroscopy (RS) offers the potential to provide point of care diagnosis of oral disease using a noninvasive, convenient, and relatively inexpensive technology. © 2014 Wiley Periodicals, Inc. Head Neck , 2014 © 2014 Wiley Periodicals, Inc. Head Neck 37 : 511–517, 2015