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Oral cavity anaerobic pathogens in biofilm formation on voice prostheses
Author(s) -
Bertl Kristina,
Zijnge Vincent,
Zatorska Beata,
Leonhard Matthias,
Schneider–Stickler Berit,
Harmsen Hermie J. M.
Publication year - 2015
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/hed.23625
Subject(s) - fusobacterium nucleatum , biofilm , tannerella forsythia , treponema denticola , microbiology and biotechnology , forsythia , porphyromonas gingivalis , periodontal pathogen , oral cavity , biology , chemistry , medicine , bacteria , pathology , dentistry , honeysuckle , genetics , alternative medicine , traditional chinese medicine
Background A polymerase chain reaction (PCR)‐based method has been used to identify oral anaerobic pathogens in biofilms on voice prostheses. The purpose of the present study was to determine the location of those pathogens inside the biofilms. Methods Biofilms of 15 voice prostheses were sampled and used to identify the oral pathogens. Fluorescence in situ hybridization was applied on smears made on glass slides and on sections of intact biofilms visualized by confocal laser scanning microscopy (CLSM). Results Fusobacterium nucleatum ( F. nucleatum ) was the most frequently detected pathogen and the only tested species detected in microcolonies. The other microbes (Parvimonas micra [ P. micra ], Porphyromonas gingivalis [ P. gingivalis ], Tannerella forsythia [ T. forsythia ], and Treponema denticola [ T. denticola ]) were not detected or only detected as single cells. CLSM analysis showed that F. nucleatum resided on the biofilm surface. Conclusion Although detectable, oral anaerobic pathogens seem to be no more than passers‐by that adhere without further observed proliferation and apparently play no striking role in biofilm formation on voice prostheses. © 2014 Wiley Periodicals, Inc. Head Neck 37 : 524–529, 2015